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Linear regression is a commonly used method to establish a quantitative relationship between two variables (e.g. the absorbance at a specific wavelength and the concentration of a particular compound). In the case of chemical analysis there is a special condition, which prohibits, for example, negative concentrations of a given substance. Knowing this fact one can determine the detection limit. The detection limit, or LOD (limit of detection), is the lowest quantity of a substance that can be distinguished from the absence of that substance (a blank value) within a stated confidence limit (generally 1%).
Many thanks to Ihor Patsay, Ivan Franko National University of Líviv, Ukraine, who contributed this linear regression tool for chemists to the Learning by Simulations Web site.